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Post by Medina on Mar 6, 2013 0:17:29 GMT
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Post by Medina on Mar 6, 2013 0:10:01 GMT
Its a explant with apical meristem and i use comercial bleach dilution of 1:2.5 and in effect, this explant was cultured in vitro after many tries.
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Post by Medina on Mar 2, 2013 15:41:59 GMT
Hi Coline, I used comercial bleach solution, it has a 5,25% v/v, diluted 1:10 (10mL of bleach and 90 mL water it is 0.52% v/v) for 10-15 minutes for seeds. For sterilisation of vegetative material I used bleach diluted 1:3 (10 mL bleach and 20mL water it is 1.75%) o bleach diluted 1:2.5(10 mL bleach and 15 mL water it is 2.1%) for 10-15 minutes with good results.
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Post by Medina on Mar 2, 2013 15:20:54 GMT
I use 1/2 MS, the plants grow well and produce root
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Post by Medina on Aug 19, 2012 20:03:38 GMT
Continuing with micropropagation of N. raflesiana, the lateral shoots are cut off and were placed in flasks with 1/3MS where they are now growing
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Post by Medina on Jun 21, 2012 20:51:24 GMT
Following with the nep work, the next photograph show N. raflesiana in vitro, from the shoot tip explant, growing in 1/3MS plus BAP 2 mg/L . The plant has more leaves and the lateral buds have begun to grow, they will be cut and put on in a new medium for get more plants. Continuing with other Nep, I cut other shoot tip from N, sanguinea, next was disinfected and now it is growing in medium 1/3MS.
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Post by Medina on Mar 25, 2012 21:41:27 GMT
Well, here is another shot tip of Nephentes raffelsiana growing in MS medium. Notice the two died leaves with the disinfection treatment. Here is the same plant with another leave come up now the plant is bigger than the beginning and show other leave.
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Post by Medina on Oct 14, 2011 22:45:17 GMT
Two years snapsnapiom.
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Post by Medina on Sept 8, 2011 20:03:29 GMT
I sown some cephalotus seeds, the sterilization was with bleach at 15%(bleach 15mL + water 85 mL ) for ten minutes. There no were contamination after three months, but none seeds germinated.
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Post by Medina on Jul 11, 2011 1:41:04 GMT
Yes coldcofee, I do not how to get it. The next step with nepenthes is add growth regulators like BAP and ANA in different concentrations and may be Kinetin too.
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Post by Medina on Jul 7, 2011 13:51:27 GMT
Good point, I add 30 g/L of sugar and PPM is difficult to get it in México, I did not use it.
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Post by Medina on Jul 5, 2011 19:18:16 GMT
tissue culture of Nepenthes growing in pots is difficult, for that I decided to try with it. After many experiments I founded a disinfection method that works for me: First, the explants are shot tips of Nepenthes plants, growing in pots with peat moss. This in one And this the other The explants were dissected, washed in detergent, soaked 1 min in ethanol, then 30 min in fungicide solution(trademark Benomil), rinsing with running tap water, then 15 min in 10 % NaOCL followed for 10 min in sterile water. After disinfection, the shot tips were placed in flasks with half strength MS medium. The results are in next photographs: One month latter, four explants from three different Nepenthes species are growing and there are not signs of biological contaminants.
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Post by Medina on Nov 3, 2009 18:45:50 GMT
You are rigth mmlr38, the concentration is the same, in both cases. I am sorry, it was a big mistake. I prepare the medium with macronutrients at 1/3 of strength, the micronutrients and organics at full concentration.
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Post by Medina on Nov 2, 2009 14:52:05 GMT
The multiplication medium is 1/3 MS with 0.5 mg/L of kinetin and it works well. If you use 0.5ml of Kintetin (1mg/ml) for one liter of medium you have too low concentration of kinetin and that explain why you get a few plants. In my case, i got ten plants for leave, in average
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Post by Medina on Oct 28, 2009 19:46:05 GMT
I had a few venus fly trap plants in vitro and i want to get more plants, for that i decide to try in vitro. The plants were take out and put it on the petry dish. Next I cut off the leaves and put them on the multiplication medium, One month later some leaves died and others turn red. Some leaves form new plants in three months. And now I have more plants of VFT.
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