|
|
Post by Medina on Oct 28, 2009 19:46:05 GMT
I had a few venus fly trap plants in vitro and i want to get more plants, for that i decide to try in vitro. The plants were take out and put it on the petry dish.  Next I cut off the leaves and put them on the multiplication medium, One month later some leaves died and others turn red.  Some leaves form new plants in three months.  And now I have more plants of VFT.  |
|
|
|
Post by mmlr38 on Nov 2, 2009 0:28:30 GMT
Nicely done! What do you use for your multiplication medium? I have most recently used 0.5ml of Kintetin (1mg/ml). In the past I used 5 ml of BAP (also 1mg/ml). I think that was a bit too strong, but I in the end I got quite a few new plants.
|
|
|
|
Post by Medina on Nov 2, 2009 14:52:05 GMT
The multiplication medium is 1/3 MS with 0.5 mg/L of kinetin and it works well. If you use 0.5ml of Kintetin (1mg/ml) for one liter of medium you have too low concentration of kinetin and that explain why you get a few plants. In my case, i got ten plants for leave, in average  |
|
|
|
Post by mmlr38 on Nov 3, 2009 0:02:43 GMT
I don't understand the difference Medina. What is the difference between 0.5 mg/L of kinetin and 0.5ml of Kinetin at 1mg/ml. Is that the same?
|
|
|
|
Post by Medina on Nov 3, 2009 18:45:50 GMT
You are rigth mmlr38, the concentration is the same, in both cases. I am sorry, it was a big mistake.  I prepare the medium with macronutrients at 1/3 of strength, the micronutrients and organics at full concentration. |
|
|
|
Post by mmlr38 on Nov 9, 2009 4:45:17 GMT
Yep, that's the same as me.
|
|
|
|
Post by julecian on Oct 21, 2010 20:25:50 GMT
hi, interesting, but, I'm interested in the leave cutting sterilization protocol, because, I've tryed some times with Venus leave cutting sterilization with a solution of Naclo3 3% x 5 minutes, and the majority of the cuttings turns black.
wich sterilization prccess did you use for this cuttings?
|
|
|
|
Post by bombsboy on Feb 22, 2011 3:04:21 GMT
they look healthy! when are you deflasking them?
|
|
