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Post by Medina on Oct 28, 2008 18:28:44 GMT
Yes, you are right , but because the seeds were in vitro I had no choice. I utilized forceps and bisturi, thus is easily break the seed coat without lost it. Thanks for your advice.
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Post by Medina on Oct 26, 2008 18:37:17 GMT
Hi. I am going to describe how I have propagated this specie in vitro Five seeds were desinfected with bleach solution (30 mL clorox plus 70 mL water) for 15 min. and washed in sterile water for 20 min. The seeds were transferred into flask with 1/3 MS medium. ¡After ten days one seed germinated! In 4 months the seedling has a shoot tip around 2 cm high and a root tip of red color. I noticed that this root had two shoot tips of green color, for that I think that the red structure has potential to propagate this specie. to propagate I take the plant out and cut off 3 new shoot tips that were growing from the red structure. Each shoot was placed into flasks with 1/3 MS medium. In this form I have now 38 plants growing in vitro. Plants to take out Plant in a petri dish Shoot tips separated Shoot tips transfered into a flask and this is when the shoot tips grow ¡¡Then the procedure could be repeated!!
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Post by Medina on Oct 24, 2008 20:30:49 GMT
Nice to meet you pananep !
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Post by Medina on Oct 24, 2008 17:20:33 GMT
Six months ago I bought 10 seeds of Drosophyllum lusitanicum, these were sterilized with comercial bleach ( 30 mL clorox + 70 mL of water) for 15 min. and washed one time in sterile water for 20 min. Five seeds were transferred into flask with 1/3MS. After six months the seeds did not germinate, then I take it out and I decide to cut 1/3 length of the seeds with a bisturi, then the seeds were tranferred into the same flasks. With this procedure four seeds germinated. seed germinating A plantula
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Post by Medina on Oct 23, 2008 19:09:55 GMT
Hi all in this forum I am a mycrobiologist but in a free time I like raising plants (Orchids, Cactus and Carnivorou plants). I have experience in in vitro plant culture. now I have in in vitro plants like: Byblis, Drosera ramellosa, D. broomensis, D. paradoxa, Dionaea muscipula and Drosophyllum lusitanicum and some species of cactus and orchids, but I have a problem, my writing in english is not good, sorry.
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