sweetpea
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MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 3, 2018 8:10:29 GMT
Konnichiwa!
First of all, I am not a Nepenthes enthusiast, but I like Nepenthes. I am quite unfamiliar with Nepenthes. In fact, I have been hardly growing Nepenthes in greenhouse. I remember the disappointed look (facial expression) of the great German Nepenthes & Heliamphora specialist, who is a worldwide distributer, when he saw a dying tiny Nepenthes (only one I had in my greenhouse at that time) in my Byblis greenhouse (see the far bottom in the page 1 of the thread: in my Byblis greenhouse) after the ICPS international conference in 2002. Back to the subject, My German friend (not the above-mentioned specialist, but he is also a specialist) kindly sent Nepenthes eymae seeds to me in 2013. I sowed the seeds in vitro, they germinated very well. Recently when I was tidying up the refrigerator, I found the remaining of the seeds without silica gel. Just curiosity, I sowed the seeds on February 9 in 2018. The seed was 5 years old. I noticed its germination a few days ago. I took the photos on March 2 in 2018. I have often heard the viability of Nepenthes seed is quite short. It might be the fact when it came to the Lowlanders? What is your experience?
Kind regards from the Far East
the germination from 5 years old Nepenthes eymae seeds
the germination from 5 years old Nepenthes eymae seeds
the germination from 5 years old Nepenthes eymae seeds
the germination from 5 years old Nepenthes eymae seeds
the germination from 5 years old Nepenthes eymae seeds
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Post by ICPS-bob on Mar 3, 2018 19:28:06 GMT
I don't have experience with Nepenthes, but I also cleaned out a refrigerator and here what I wrote in CPN in 2012: While cleaning out an old refrigerator in the lunchroom at my office, I discovered a paper bag containing packets of seeds of Drosophyllum lusitanicum, Byblis gigantea, and several Drosera species from the ICPS Seed Bank that had been there for 22 years! As you can imagine, the seed storage conditions in an office lunchroom refrigerator are less than ideal. The paper bag and seed packets were stained with all sorts of unknown spilled food stuff. In late March, I scratched the seed coat of 100 of the 22-year-old Drosophyllum seeds on fine sandpaper until I saw the white endosperm. These scarified seeds were then soaked in tap water for 24 hours. I put the 100 seeds on the surface of moist vermiculite to see if they would germinate. To my great surprise, the first seeds began to germinate in 13 days. At the end of two months, 76 of the 100 seeds had germinated (Fig. 1). I sprinkled the old Byblis gigantea seeds on the surface of a wet mixture of equal parts Canadian milled sphagnum peat and silica sand in a glazed ceramic pot. Two days later, I put a handful of dry grass on top and burned it. The seeds began to germinate within 35 days. I did not count the number of small seeds or estimate germinate rate. I was just surprised that the 22-year-old seeds germinated. None of the old Drosera seeds germinated. legacy.carnivorousplants.org/cpn/articles/CPNv41n4p154.pdf
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 3, 2018 20:04:00 GMT
Dear Bob-san, (As you know I prefer to call you Z...-sennsei)
Konnichiwa!
Great information!!! I also have many old cp seeds in the refrigerator. Your experience encourages my future trials. Thank you very much Bob-san!!! A few members of other forums have doubt about the ID (genus) of the seedlings. Bob-san, please believe the seedlings are definitely Nepenthes.
Kind regards from the Far East
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 5, 2018 16:17:49 GMT
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 6, 2018 12:31:01 GMT
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 13, 2018 14:11:51 GMT
While waiting for the update02, I am not sure it is an appropriate thing species being categorized as Lowlanders, Intermediate or Highlanders, so far as the seed viability. But, among Lowlanders, the appearance of N. northiana seed is significantly different from N. ampullaria. We probably should consider to separate N. northiana from the amp/raff/bical group. What do you think of it? I am trying to grow N. northiana from a few sourses. I feel N. northiana seed viability is a bit longer than I expected. Another N. northiana seeds I obtained in April, 2016. I sowed them in the plastic container immediately. They started germinating on May 02 in 2016. I sowed the remainder of them in vitro on April 10 in 2017. I had stored the seeds in the refrigerator, no silica gel. I did not remove the seed husk in this case. They started germinating on June 10 in 2017. Please see attached photos, you can see the seed husks. Although they are neglected, they still look healthy, imo..... I took the photos on March 13 in 2018. Kind regards from the Far East
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 15, 2018 14:05:01 GMT
update 02 About storage, My thought for the moment, 1: the seeds should be high quality in the first place. 2: cold storage is necessary. 3: keep or control the seed moisture content (IMPORTANT) 4: inhibit propagation of bacteria and fungus 5: something I have no idea
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 23, 2018 11:54:20 GMT
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 24, 2018 0:05:06 GMT
update 03-plus
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Mar 28, 2018 13:33:45 GMT
Konnichiwa! While waiting for the update04, I took all photos on March 28 in 2018. I have been confused about the opinion that is the cold storage damages Nepenthes seeds. The other experiences that contradict this opinion, though much shorter period than N. eymae (storage for 5 years in refrigerator). I sowed N. aff. truncata seeds in a plastic container in August 2016, though they still have been neglected in the plastic container. I stored the remainder of the seeds into the refrigerator, with no silica gel. I sowed them in vitro on March 6 in 2017 (> 6 months old). I transplanted them once. Please see attached photos. N. aff. truncata in vitro(from the seeds stored for > 6 months in refrigerator) N. aff. truncata in vitro (from the seeds stored for > 6 months in refrigerator) N. aff. truncata in vitro (from the seeds stored for > 6 months in refrigerator) I got N. peltata seeds from my friend on March 28 in 2017. I sowed almost N. peltata seeds in vitro on March 30 in 2017. Please see attached photos Nepenthes peltata in vitro Nepenthes peltata in vitro Nepenthes peltata in vitro The seed appearance resembled, if anything, N. northiana & N. suratensis. Please see attached photo The seed husk of N. peltata. I stored the remainder of seeds (very small numbers) into the refrigerator, with no silica gel. I sowed them in the plastic container on February 9 in 2018 (10 months old, stored for 10 months in refrigerator). Please see attached photos. Nepenthes peltata (from the seeds stored for 10 months in refrigerator) Nepenthes peltata (from the seeds stored for 10 months in refrigerator) Nepenthes peltata (from the seeds stored for 10 months in refrigerator) Nepenthes peltata (from the seeds stored for 10 months in refrigerator) Nepenthes peltata (from the seeds stored for 10 months in refrigerator) My question is “How long does the cold storage damage Nepenthes seeds?”. Kind regards from the Far East
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sweetpea
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MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Apr 2, 2018 15:04:18 GMT
Update 04 Konnichiwa! If you agree to my claim that these seedlings are Nepenthes, please share your experiences or your opinion about "How old is too old?". I think nobody denies your experience even if it would be contrary to common belief. At least I never deny your experience. Kind regards from the Far East I took all photos on April 02 in 2018. Nepenthes eymae (from the seeds stored for 5 years in refrigerator) Nepenthes eymae (from the seeds stored for 5 years in refrigerator) Nepenthes eymae (from the seeds stored for 5 years in refrigerator) Nepenthes eymae (from the seeds stored for 5 years in refrigerator) Nepenthes eymae (from the seeds stored for 5 years in refrigerator) Nepenthes eymae (from the seeds stored for 5 years in refrigerator)
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Apr 4, 2018 14:16:57 GMT
Konnichiwa! Is there anybody being familiar with the technical terms? I call what I removed the Nepenthes seed husk. Some members on TerraForums, where I am stubborn, arrogant, and.... , call it the Nepenthes seed coat. TerraForumsThe membrane that I provisionally call the seed coat still wraps the actual seed, except the one end where the root will be emerging. It still seems to protect the seed. I illustrate it by using the peanut though it is improper. Could anyone explain the technical terms? Kind regards from the Far East Nepenthes eymae: the part that put a red ring around, the membrane doesn't exist originally there. Peanut: the part that put a red ring around, I artificially removed the coat.
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sweetpea
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MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Apr 9, 2018 11:57:14 GMT
Konnichiwa! Jan-san (Dr. Jan Schlauer), who has been a good friend of mine since I joined the cp-listserve in 1994, answered my question. ...... Regarding the seed coat, this is the commonly used term. The external form and the mechanical properties of the seed are defined by the outer layer that is called testa. Additionally the embryo is included in the inner (protective) layer that is called tegmen and is a thin membranous peel in Nepenthes. Other seeds have endosperm that additionally surrounds the embryo, but in Nepenthes the endosperm is not well developed or lacking whatsoever. ...... Kind regards from the Far East Konnichiwa! Is there anybody being familiar with the technical terms? I call what I removed the Nepenthes seed husk. Some members on TerraForums, where I am stubborn, arrogant, and.... , call it the Nepenthes seed coat. TerraForumsThe membrane that I provisionally call the seed coat still wraps the actual seed, except the one end where the root will be emerging. It still seems to protect the seed. I illustrate it by using the peanut though it is improper. Could anyone explain the technical terms?
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sweetpea
Full Member
MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Apr 12, 2018 15:05:45 GMT
Konnichiwa! I took the photo on March 06 in 2018. After I took the above photo, I sowed these seeds. It seems the old seedlings have the strength to push through their seed husk. I took the following photos on April 12 in 2018. The germination from 5 years old Nepenthes eymae seeds (stored for 5 years in refrigerator) The germination from 5 years old Nepenthes eymae seeds (stored for 5 years in refrigerator) The germination from 5 years old Nepenthes eymae seeds (stored for 5 years in refrigerator) Though I don't understand the conditions for the appropriate storage of the Nepenthes seeds, it seems viability among Nepenthes seeds is a lot longer than what we may think and have/may read, as N_CloudySkies-san said on TF ( page4). Kind regards from the Far East
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sweetpea
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MOLLIE RILSTONE
Posts: 163
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Post by sweetpea on Apr 12, 2018 21:29:04 GMT
Update 05 I took the photos on April 12 in 2018. The germination from 5 years old Nepenthes eymae seeds (stored for 5 years in refrigerator) The germination from 5 years old Nepenthes eymae seeds (stored for 5 years in refrigerator) The germination from 5 years old Nepenthes eymae seeds (stored for 5 years in refrigerator)
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