Using Antibiotics In Vitro

[nlegaux]

In all of my cultures that I have used leaf cuttings in (from both D. muscipula and D. capensis), I have had no contamination except one contaminant that i can't seem to get rid of. The only explant treatment I have found that prevents this contaminant kills the explant. I have identified the contaminant as some sort of bacteria, and I would like to know what kind of antibiotics you would recommend using. My media is comprised of 1/3MS, 6g/L agar, and 24g/L sucrose. 

 



Sorry for the poor quality of the photo. The contaminant looks like a dark haziness that spreads away from the cuttings, and is normally visible about 24 hours after I make the culture.

Thank you in advance,
Nick

[coline]

Those are phenolic exhudates, normal for a VFT ater a dissinfection protocol, a frequent medium change or adding some activated carbon to it helps the plant overcome them

[nlegaux]

Thank you for the response. How much activated carbon would you recommend adding?

Thank you,
Nick

[coline]

500mg/L is a normal amount