coline
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Life's essence: patience
Posts: 484
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Post by coline on Mar 6, 2013 22:05:57 GMT
I was thinking in seeds as the drosera seeds, such tiny objects are no easy target to pick even with the smallest tweezer. What are your methods for taking the seeds from the dissinfection treatment to the culture media?
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Post by adamcross on Mar 7, 2013 8:31:49 GMT
Minute seeds can be sterilised effectively in fine gauge nylon mesh bags (10-20 um; I use heat sealed mirrorcloth), then washed in sterilised water and transferred onto culture media in a sterile area such as a laminar flow. If you don't want/don't have the facilities to do this, you can try using sterile filter paper to wick or blot the seeds from the surface of the sterilisation treatment. You then still need to wash the seeds without losing them somehow however.
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coline
Full Member
Life's essence: patience
Posts: 484
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Post by coline on Mar 7, 2013 12:42:39 GMT
So them with this methods, you would even not count specifically how many seeds do you place in the jar, or is it possible?
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Post by adamcross on Mar 7, 2013 15:18:02 GMT
If I need to count the seeds specifically, I use a pair of fine needle-nosed forceps to count out the required amount on a white porcelain tile. The shiny surface of the tile makes counting easy, and as it's not plastic the seeds don't build up a static charge as readily and fling themselves all over the room on a whim. Once counted you can just scrape them off the tile into the mesh bag using a small funnel.
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coline
Full Member
Life's essence: patience
Posts: 484
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Post by coline on Mar 7, 2013 22:11:19 GMT
Thanks, I will try the technique and see how it goes!
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