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Post by mcmcnair on Oct 27, 2011 18:17:15 GMT
I am an undegraduate student at NC State University as well as an avid carnivore enthusiast and I have recently been lucky enough to start a new undergraduate research project where I will be attempting to induce mutations in Sarracenia species and hybrids by way of colchicine, surflan, and radiation. We are hoping to work with the following plants specifically:
S. purpurea ssp. venosa, S. flava, S. rubra ssp. rubra, and S. minor; S. purpurea ssp. venosa var. montana, S. rubra ssp jonesii, S. rubra ssp. wherryi, S. oreophila; and S. x catebaei, S. x popei, S. x harperi, S. chelsonii
We are hoping to begin the experiment this coming spring, the biggest problem being that we do not have any of the plants or seeds that we need. If anyone has any seeds or adult plants that they would be willing to donate we would greatly appreciate it. After the experiment all the plants will be donated to the Carnivorous Plant display here at the NC State Conservatory. If you have any questions or would like to contact me, please email me SarraceniaMutation@gmail.com
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Post by Brian Barnes on Oct 27, 2011 20:40:41 GMT
Hello! The ICPS would be happy to assist you in your research. Please send me your school's contact info. I'll be happy to send you seeds from a few type locales of Sarracenia, all from my own plants of course. In exchange, might we request a small article from you for our quarterly CPN journal?  Happy Growing, Brian Barnes ICPS Director of Conservation |
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Post by ICPS-bob on Oct 27, 2011 23:17:42 GMT
Sounds like an interesting project. Are you planning to work with seeds or mature plants. Will your treatments involve tissue culture? I hope you realize it will take a bit of time to observe results. As I am sure you are aware, Sarracenia grow rather slowly.
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Post by mcmcnair on Oct 28, 2011 5:51:21 GMT
Currently we are planning on working with seeds only, however we have discussed using pollen which would require us to have mature plants to both gather the pollen from and to cross with. Also, working with seeds tends to be a little cheaper. Ideally, we would like to work with the following species and hybrids: S. purpurea ssp. venosa, S. flava, S. rubra ssp. rubra, and S. minor; S. purpurea ssp. venosa var. montana, S. rubra ssp jonesii, S. rubra ssp. wherryi, S. oreophila; and S. x catebaei, S. x popei, S. x harperi, S. chelsonii. Currently we are not planning on using tissue culture, mainly because of a lack of funding, resources, and experience in TC-ing sarracenia. I've been growing Sarracenia since I was little so I'm used to waiting on the seedling to develop. I've got 2-3 years left of college, not including grad school so i'm not too concerned with the time it takes to grow them out. And I would gladly write an article for the CPN in exchange for any seeds and help provided  |
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Post by mcmcnair on Nov 3, 2011 15:52:05 GMT
We have decided not to use hybrids in this experiment because of the difficulty of acquiring the seeds. Therefor, we have decided to use a few more varieties. the new list of plants being used in this experiment is as follows:
S. purpurea ssp. venosa var. montana, S. purpurea ssp. venosa var. burkei(rosea), S. purpurea ssp. venosa
S. flava, S. flava var. maxima, S. flava var. rugellii
S. alata
S. minor
S. oreophila
S. rubra ssp. rubra, S. rubra ssp. jonesii, S. rubra ssp. wherryi
thanks for the help!
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Post by Brian Barnes on Nov 11, 2011 22:22:39 GMT
Seeds are on the way to the address you provided, compliments of the ICPS! Keep us updated on your experiments... Happy Growing, Brian |
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Post by mcmcnair on Nov 15, 2011 18:21:32 GMT
We are still short seeds for all the Sarracenia except S. minor, and S. rubra ssp. wherryi
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Post by mcmcnair on Jan 10, 2012 21:06:15 GMT
My project partner and I would like to thank you all for the donations! We now have all the seeds we need. I'll keep updating you all as the experiment proceeds.
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Post by mcmcnair on Feb 5, 2012 0:23:38 GMT
Yesterday, Feb. 3rd, 2012, we put all of our seeds into stratification using the Meadowview method. For those of you that are not familiar that means putting the seeds into a paper towel, twisting it into a sort of sack, dampening the towel with DI water, putting the paper towel sack into a ziploc bag with plenty of air, and finally putting the bags into a cooler set at 4C. Every week we will switch the seeds to a freezer set around 0C for a week then back into the cooler they go. They will be in stratification for five weeks. Then we will be doing all of our treatments.
On another note we have decided to change the oryzalin and colchicine treatments so that instead of soaking the seeds in the chemicals we will be doing spot treatments on the crown of the seedlings after the first true leaves emerge. The radiation treatments have not changed.
We decided to only use ten species/varieties for our experiment. We are using S. alabamensis ssp. wherryi, S. alata, S. flava, S. flava var. ornata, S. jonesii, S. minor, S. oreophila, S. purpurea ssp. venosa, S. rosea, and S. rubra ssp. rubra. Three species will get radiation and chemical treatments; minor, oreophila, and rosea. The other species will all get chemical treatments only.
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Post by ICPS-bob on Feb 5, 2012 1:14:11 GMT
What was the basis for your decision on treatments?
radiation and chemical treatments: minor, oreophila, and rosea (a type of purpurea)
chemical treatments only: S. alabamensis ssp. wherryi (a rubra), S. alata, S. flava, S. flava var. ornata, S. jonesii (a rubra), S. purpurea ssp. venosa, and S. rubra ssp. rubra [in other words 3 rubra types, 2 flava types, alata, purpurea].
Where is your replication? In a normal replicated experiment, you would divide your 10 types of seed into 3 partitions (3 x 10 = 30) and select treatments as: 10 Control (no treatment), 10 chemical treatment, 10 radiation and chemical treatment.
Without replication, you will be unable to separate treatment effect from species effect. Assuming replication, without a no treatment control, you will be unable to determine treatment effect (other than the possible effect of radiation and chemical vs no radiation); you will be unable to say anything about chemical treatment effect -- since both sets were chemically treated.
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Post by mcmcnair on Feb 5, 2012 1:48:57 GMT
the basis for decision for our treatments is that we had more seeds available for minor, oreophila, and rosea than we did of any other species. We have 500 of those, and only 250 of the other species mentioned.
There is a control group of fifty seeds for each species. The three treatments are broken down into groups of fifty seeds for each sub-treatment. For the radiation, we will be irradiating the seeds at five different Gy levels, so five subtreatments. There are 250 seeds being used for the radiation treatments. Each level of Gy unit treatments will be evaluated in groups of ten seed so that is five repetitions of each subtreatment plus a control that has no treatments. The chemical treatments in total contain 200 seeds and are broken down into four subtreatments of 50 seeds each. For evaluating the chemical treatments they will again be divided into groups of ten seeds, resulting in five replications. Sorry I didn't make that clear. Does that clear everything up?
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