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Post by bigbella on Oct 10, 2011 3:41:27 GMT
Here are a couple of shots of some highland Nepenthes seeds within a few days of their germination in 1:3 MS media. At the left, the radicle and hypocotyl are visible, just emergent; and the cotyledons can be seen, just unfolding, at the right. Nepenthes sp. -- 9 October 2011
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Post by bigbella on Feb 19, 2012 19:30:04 GMT
Here is an update on one of the highland Nepenthes species illustrated at the beginning of this post; and I am still getting new germination almost six months out. The most successful seed batches to date were kept under highland conditions from the very start: relatively warm days (as high as 24˚ C) and cold nights (as low as 10˚ C) . . . Nepenthes sp. "A" 15 February 2012Here is one of those same cultures several, now months removed. Some Nepenthes species grow like weeds in vitro; then there are others that, while clearly outpacing that seed planted in compost, still remain on the slower side . . . 10 July
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Post by bigbella on May 14, 2015 20:17:37 GMT
Here are a few images of multiplying plantlets in a cytokinin-rich experimental media. The challenge with this species, as well as many other highlanders and ultra-highlanders, are the development of roots; or rhizogenesis. More often than not, I allow the plants to root ex vitro, as though they were cuttings; it's far cheaper, in terms of labor and materials, and often quicker in the long run . . . Nepenthes villosa "Gamma Clone" (Tambuyukon) 18 AprilNepenthes attenboroughii (Mt. Victoria)
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coline
Full Member
Life's essence: patience
Posts: 484
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Post by coline on May 17, 2015 16:29:15 GMT
I have done the same, only that I have seen very much that plants really take up incredibly quickly if they have a good root system when taken out, sarracenia, heliamphora and nepenthes all behave the same. Only that what I do is to grow them on plain media for some time (1 replate) before taking them out, have not tried adding IBA.
Have you had anytime an algae contamination problem? one of the seeds (very rare) that I got had algae growth, and almost all if not all of the seedlings developed algae growth, you see all the green spots growing around them.
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Post by bigbella on May 18, 2015 7:19:41 GMT
I have done the same, only that I have seen very much that plants really take up incredibly quickly if they have a good root system when taken out, sarracenia, heliamphora and nepenthes all behave the same. Only that what I do is to grow them on plain media for some time (1 replate) before taking them out, have not tried adding IBA. Have you had anytime an algae contamination problem? one of the seeds (very rare) that I got had algae growth, and almost all if not all of the seedlings developed algae growth, you see all the green spots growing around them. My main reason for often eliminating Stage III (rooting), are that some highland Nepenthes are terribly slow to produce roots, whether or not they've been transferred to plain media; or media with auxins. I have had consistently positive results, simply placing them in composts if milled sphagnum or live moss. On rare occasions, I have had seed batches which had been contaminated by algae; though some cultures have survived serial cleanings without too many problems . . .
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coline
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Life's essence: patience
Posts: 484
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Post by coline on May 18, 2015 11:50:47 GMT
what have you used to clean them?
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Post by bigbella on May 19, 2015 3:30:58 GMT
what have you used to clean them? I have used serial rinsing with sterile distilled water; replating on new media, along with silver based anti-algals . . .
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coline
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Life's essence: patience
Posts: 484
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Post by coline on May 19, 2015 15:21:19 GMT
Thanks, I will try rinsing while I find the silver anti algae products, have never heard of them, where are they sold?
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Post by bigbella on May 20, 2015 6:12:14 GMT
Thanks, I will try rinsing while I find the silver anti algae products, have never heard of them, where are they sold? The product that I had used was called "FungiGone," and was distributed by Bioworld; and was said to contain silver ions as well as some proprietary component. If I recall, it had to be added -- sterile-filtered -- to media, post-autoclave . . .
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coline
Full Member
Life's essence: patience
Posts: 484
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Post by coline on May 20, 2015 20:57:24 GMT
Thanks, found it!
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Post by bigbella on May 20, 2015 22:51:06 GMT
Hi, You may able to get a free sample from them, if you inquire. I did, when I was researching using their product line . . ,
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coline
Full Member
Life's essence: patience
Posts: 484
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Post by coline on May 21, 2015 20:25:08 GMT
But it requires to be delivered to a lab establishment? when I asked them about information they said I needed to provide "company name" and other details
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Post by bigbella on May 21, 2015 21:19:44 GMT
But it requires to be delivered to a lab establishment? when I asked them about information they said I needed to provide "company name" and other details Just give your lab a fictitious business name, something like Yoyodyne Propulsion Systems. That's what I used in 2013 . . .
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Post by killerplants4realz on May 22, 2015 1:11:59 GMT
Yoyodyne is the name of my company what a cawinky dink;)
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Post by killerplants4realz on Aug 26, 2015 14:07:06 GMT
Bigbella are those colored circles labels? The stickers at the bottoms of the TC containers? Must be the date or what hormone you are using? Thanks. Love the TC pics happy growing to you and coline!!!
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