VFT In Vitro

[eric]

I started TCing in January. I've had minimal success but success none the less. The only thing i've seemed to be successful with, as far as CPs, is venus flytraps.

Dente strikes



Typical with several plantlets



Seedlings germinated in vitro

[peterhewitt]

Can you give us a rundown of your sterilization protocol?

[eric]

For dionaea seeds I simply use a 1/10, bleach/water mix. Soak them for about 10 minutes, don't rinse them and straight onto media. I use a small dropper to remove excess liquid from the media. Very easy and the bleach thats not rinsed doesn't seem to effect the seeds growth at all.

[peterhewitt]

I am more interested in your procedure for explant initiation.

[eric]

For explants I use different procedures everytime because i'm still trying to find what works best for me. For the Dente I used a 1/10 bleach water solution, rinse in sterile ro water, 50/50 solution of peroxide/water, rinse, dip in isopropyl alcohol, rinse and onto media. Sorry but I can't remember the exact times but i'm sure it was over 5 minutes in each solution except for the alcohol in which I only give them a quick dip. The explants are all rinsed prior to sterilization in tap water with a lil dish washing liquid.

I'm def just a noob though and any tips or suggestions are very welcome. Thanks for stopping by Peter!

[eric]

Heres a small update .

Dente traps



Drosera adelae



Drosera capensis 'All Red'



Typical venus flytrap



VFT seeds



Cephalotus explant



VFT seedlings

[peterhewitt]

Hey Eric, you seem to have a handle on it. Have you experimented with PPM yet? I am in the process of initiating a number of Dionaea variants, and am also looking for the best Protocol.

[eric]

Hi Peter. Yes, I use 1ml of PPM per liter of media. I'm sure you'll be able to tackle it. If I can anyone can lol. Good luck!

[eric]

Small update

N. truncata thats been in vitro for a couple of months with no growth



Ceph explant still looking good after almost a month in vitro



Typical VFT explant



N. ventricosa tendril still living with no contams after about a month

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Dente explant callusing from the leaf and inside the trap





Growth on a Bird Of Paradise seed in vitro



Thanks for stopping by .

[Robert]

Hello Eric, what is the response of the ventricosa tendril?

[carniplants]

Good, job, mate! Let's see how the Cephalotus expland does. Well done!

[coline]

Eric, what are your medium ingredients or concentrations you used?

Also, how is your cephalotus explant by now?