Since I did not kill the seeds, and I see germination, I have used 1/3MS with 8%PPM for 7 hours in highland seeds as the only dissinfection process. And for tissue I followed Medina's protocol mentioned in this forum with 80% success.
Well no, NaClO (as it is written correctly) is the common bleach, as it is a liquid, you dilute 10ml in 90 of water, try to see if they use 3,5% active ingredient or 5,25% (which are the most common concetrations sold) what I use is 5,25% a.i.
Well, Benomyl indeed affects, but more sensitive species, as Sechium edule or maybe others, but with nepenthes, even with repeated dissinfections through it, has had no effect, or.. it may have helped shoot multiplication.
PPM of course is not for sale in Korea, it is normally not sold anywhere, you would need to buy it from www.ppm4plant-tc.com/ (in some cases they may send a free sample) Or from kitchenculturekits.com
here is the latest media formula (always trying to improve it)
NEPENTHES BASAL SALT MIXTURE and add full strength micronutrients 1 lt of distilled water
after you make this use 50 ml of above mix and add 2ml of PPM this will give you a good sterilizing treatment it works well Soak seed 4 to 24 hours in this don’t rinse just place seed strait on to media
the 1Lt of media you have made I add: 0.5 ml of PPM 25g sucrose (sugar) add 7.5 grams of growgel or about 5 grams of AGAR (test this out first with some plain water as some brands of agar require different ratios) adjust PH to 5.5 - 5.68 range (use white vinegar to lower PH and bi-carb soda (baking soda) to raise PH) Add media to your containers or jars and sterilize as normal. I have started to reduce the amount of PPM I use in my media as it is starting to show its limitations as an anti fungal treatment. plants with no signs of contamination suddenly start to become contaminated after a few months. Its seems to hide contamination rather than prevent it. For shoot multiplication use above media with 0.2mg/liter BAP and 0.2mg/liter Kinetin For callus use the same media with 0.2mg/liter BAP and 0.1mg/liter of TDZ. (TDZ is the way to go for callus production) for shoot formation use 0.5mg/liter of NAA