seeds disinfection

[Medina]

I had used the next method for seed disinfecting with success:

1) With a piece of filter paper make an envelope( 3 cm X 3cm ), put the seeds (no more than 50) in the paper. fold it in two, fold the ends and staple it.




2) Prepare a 20% solution bleach (20 mL bleach + 80 mL water), add two drops of liquid detergent and fill a flask.



3) Put the envelope with seeds into the flask, mix and wait 10-15 minutes.


4) Now take the envelope out, with sterile forceps and put it into another flask that contain sterile water, mix and wait 15 minutes. in the photograph the flask with red lid is sterile water and the another is solution bleach

5) Take the envelope out with sterile forceps and put in a sterile petri dish. In this case I put the envelope over the lid, but it is better the petri dish. With sterile forceps and scalpel quit the staple and open the envelope, using forceps transfer each seed into the flask with medium.

With this method I have germinated seeds like:
D muscipula red form


D. broomensis


Byblis


D paradoxa


D ramellosa


and D lusitanicum


That is all, Enjoy it.
---------------
ADMIN: I removed 2 images that had nothing to do with this article or CP. One of them greatly exceeded the size guidelines.

[mmlr38]

Very nice! Thanks for sharing.

[pygmydrosera]

Hi,

Please can you tell me how long is normal for the seeds to germinate on the agar ?? I have also did this with seeds 15 min sterilization and I did this on 14-06-2011 and now 30-06-2011 nothing happend yet :-( So I hope they will still germinate

[peterhewitt]

It depends on what seeds they are. It could take anything from two weeks to months, depending on freshness of seed and species. If you used PPM in your media it could take longer than expected.

[pygmydrosera]

The seeds where seeds from Petiolaris complex drosera and Cephalotus seeds. Please can you tell me what you mean with PPM ??

[peterhewitt]

PPM is Plant Preservative Mixture. It is a broad spectrum anti Bacterial agent, used to suppress bacteria in difficult to sterilize material.

[peterhewitt]

Both Cephalotus and Petiolaris Drosera can take quite some time to germinate. It could take six months or more for Cephalotus.

[pygmydrosera]

thank you very much for these info Peter.

So when I have sterilize my petiolaris complex drosera seeds and cephalotus seeds for a few seconds in 70% isopropylalcohol and after that for 15 min inside a 1% bleach and then 3 times wached with sterile water is good ? That's what a company here in my country give me as advice before putting the seeds on the sterile agar media.

[peterhewitt]

That sounds good, but you should do batches of seed at different times in the bleach. I use a 10%bleach solution for 5 min or sometimes a little more. But using less bleach for longer is a safer way to go.

[coldcoffee]

I will have to try this with drosera seeds!
I recently, following 2 failed attempts at traditional germination, decided to try germinating Camellia sinensis (Tea- as in the plant producing the tea we drink) in-vitro. At the time I used the same media for a few other species as well which have shown no signs of problems thus far (among them, S. oreophila, S. jonesii and Panax quinquefolius- American Ginseng). The Camellia sinensis is horribly contaminated! I think the problem lies in the size of the seed. Camellia sinensis seeds are fairly large compared with, say S. oreophila or even Panax quinquefolius. If I try again, I will likely use a higher concentration of disinfection solution, or will let them soak longer (and agitate more).

With the remaining Panax quinquefolius and Camellia sinensis, I decided to try a little experiment and germinate them in pure vermiculite.

[coldcoffee]

P.S. Love the Byblis picture!!

[DroseraBug]

Have you had good results using this method with Sarracenia seed? Your first photo resembles Sarracenia seed.

[bigbella]

In the past, I have used bleach and vinoxide solutions to sterilize the seed; but now I simply use an 8% mix of PPM to 1/3 MS without any pH adjustment and soak the seed for three to four hours. The seed is placed, without rinsing, upon the media . . .

[snapsnapiom]

great read

I have been successful at doing VFT seeds very similar to the posted also capensis seeds aswell

The one question i have in the photo of the flytrap that you said you grew from seeds how long did it take to get to that stage please ?

[Medina]

Two years snapsnapiom.