matti
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Posts: 216
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Post by matti on Jul 25, 2007 11:22:46 GMT
;D Just testing to see if the media worked.  And it did, mold!.  |
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Post by jm82792 on Jul 27, 2007 0:46:17 GMT
Sorry about that,what helped for me is PPM you may be able to get a 10 mili liter trial size. The you can only get cotamination from the from the explant that is what I had.
Alas I have no pressure cooker or microwave so I'm on a halt..........................
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Post by pitcherfreak on Jul 28, 2007 6:17:51 GMT
If its just for cooking TC media you could try getting a second hand one cheap always get tyhem to prove it works if you are getting a microwave. Get them to boil/heat a cup of water for you.
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matti
Full Member
Posts: 216
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Post by matti on Jul 28, 2007 7:53:41 GMT
No microwave? Do you live in a tent aswell? microwaves are about 100bux now. JM I think you need to read my first post more carefully, I was just testing to see if I could grow mold on the media and I obviously can. I threw a capensis leaf in to see what would happen, so tonight I may attempt the real deal. Here is a photo of moldworld, I have about 5 different forms growing in there. ;D ;D ;D  |
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Post by phissionkorps on Jul 29, 2007 7:55:14 GMT
Actually, from your post (especially when coupled with the title), it seems as if you were trying to TC the capensis and failed via the mold.
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matti
Full Member
Posts: 216
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Post by matti on Jul 29, 2007 8:15:04 GMT
Please read the post before mouthing off with a subtly snide and openly hostile comment. I think I would know what I was trying to attempt more than you.  . Don't try to perceive me as most of the time I am usually thinking the opposite thing. |
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Post by phissionkorps on Jul 29, 2007 19:22:25 GMT
"Mouthing off"? Are you kidding me? Who do you think you are?
As much as you're totally not worth me wasting my time or energy to engage....
I did read the post, and I'm just telling you that's what it seemed like to probably everyone else but yourself. Only you would get "offended" by a comment like that. I just think it was pretty ridiculous to snap at jm for something that you didn't make clear until after you snapped at him for not understanding.
Sorry Mr. Enigma...guess I'll have to ask permission or something next time.
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Post by ICPS-bob on Jul 29, 2007 19:36:31 GMT
Matti and phissionkorps, cool it. We do not want this type of interchange here.
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Post by jm82792 on Jul 29, 2007 21:43:27 GMT
You need to stare at the microwave,my mom does not like that  I know alot about microwaves they don't iradiate your brain, so a pressure cooker is my only option. Try the real deal though you will have some success. |
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Post by Deleted on Jul 30, 2007 0:01:29 GMT
microwaves are not that expensieve and they last along time i just got a new microwave this weekend after my last one caught on fire after having it for 3 years and my new one only cost 49 dollars at best buy here in california
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agar
Full Member
Posts: 23
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Post by agar on Jul 30, 2007 6:13:08 GMT
Guys, don't sweat it... "contamination" happens;-)
For your benefit... I use a small kitchen pressure cooker. Microwaves are great for heating up leftovers and such, but seem too "fast" for processing media. I recall (and by the way still have) the first microwave my mother bought in the 1970's. I vividly recall "warming up"a leftover steak, and ending up with (literally) a piece of charcoal! I decided from the get-go with this TC stuff to not use the microwave to prepare media. Not that it can't be done, but it's just too "iffy".
The somewhat slower approach with a pressure cooker (~15PSI for around 15 minutes) works well, and is lengthy enough to consistently "dial in" the right amount of agar to get the desired viscosity of the media.
Regardless of the media prep, what mostly gets you into trouble is the actual transfer of explant material into the containers. It has to be sterile and you're work area has to be clean, and you have to be clean and efficient as possible, or the contamination rate will be high. Experimentation and experience will be the key here.
You can get by with non-lab conditions and equipment, but you have to be as careful as possible not to introduce any air born contaminants into the cultures as you can manage, and try to transfer material in as "clean" an area as you have available.
Hope this helps,
agar
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Post by Michael Catalani on Jul 30, 2007 15:51:15 GMT
I've used a microwave with great success. I was going to get a pressure cooker, but have found the microwave serves well enough. It helps if you prepare media in standard sizes so that you can get your timings down pat. For example, if you prepare one liter of media, this will handle about 22 baby food jars. Each jar must contain EXACTLY the same amount of media in order for each jar to heat up at the same rate. Now that I have the timings down for 22 jars of identical amounts of media, I prepare all of my batches in 22 jar quantities.
The most frequent source of contamination for me is the initial culture, which simply was not cleaned well enough. After getting a culture established, the rate of contamination drops significantly for me in subsequent transfers.
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Post by jm82792 on Jul 31, 2007 3:51:25 GMT
Well I want to buy a stainless steel 8 quart cooker
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Post by pitcherfreak on Jul 31, 2007 7:29:27 GMT
Should work well for you JM just make sure before you buy it it will fit the containers you are going to sterilize the media in. Good luck with the hunting. If you are short on cash I know I've occasionally seen pressure cooker in auction marts.
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Post by jm82792 on Jul 31, 2007 18:45:43 GMT
I have 25 autoclave baby food jar caps,
it is not a big priority but I'm going to still look
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And it did, mold!.
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I know alot about microwaves they don't iradiate your brain, so a pressure cooker is my only option. Try the real deal though you will have some success.
