|
Post by jm82792 on May 21, 2007 2:11:08 GMT
Okay I have TCed some things so in a week pictures will come I hope 50% of them make it and I get better % with experience. All it is is venusta,capensis(yes a weeds I know),esseriana(mexi ping) and a red vft. When you think you have everything you have to forget something like I did for instance the pH was to be 5.8 not 6.8 so I had to fix that,did not add enough agar and had water instantly vaporize (explode)! So a check list is to be needed ,if some of the cultures work and you want to know my mistakes I can post them all up. (Water explosion scroll down)
|
|
Clint
Full Member
Posts: 808
|
Post by Clint on May 21, 2007 2:21:36 GMT
Oh you aren't using an autoclave?
|
|
|
Post by Sean Spence on May 21, 2007 2:36:39 GMT
Are you using seed or plant material?
If you are using parts of plants, how did you sterilize them?
|
|
|
Post by jm82792 on May 21, 2007 4:36:22 GMT
I am using the microwave I am nuking until boil then waiting to cool then I do a alcohol dip,10% bleach and 2-3 5 minute rinses. I hope this works I did use PPM biocide so I hope at least one works. I used BAP hormone for the media it is shoot inducing,I used this site for the steps and the sites owner helps me on the forum. www.world-of-carnivores.com/tissue_culture.htmlI have 20 liters of media so I can try alot of times. I basicaly did the same thing as the kitchen culture kit but I spent less money and substituted some things......
|
|
|
Post by endodoc on May 22, 2007 12:04:50 GMT
jm82792 glad to see you are taking the plunge into TC; keep posting, many of us newbies will benefit from your posts, the forum you are referring to is excellent, a bit much for my brain regards ed
|
|
|
Post by jm82792 on May 30, 2007 5:29:24 GMT
Okay 50% of the explants turned black from contamination while the rest are fine, My media preperation was perfect because the contamination oreginates from the explants and the PPM kept the contamination from spreading much so it took a while to notice it. I did work when there was a draft and my momwas packing stuff. So in perfect conditions I may get 90%.
|
|