Post by richardhole on Oct 15, 2016 6:47:20 GMT
Hi
Do you know anyone that has tried honey in the tissue culture medium or are you able to try it? It kills bacteria and is good for plants and striking cuttings. I have used it to strike cuttings. Honey may increase the chances of the plants in culture living without infection and it may save a lot of time and expense of the extra sterilization. I thought it would be a low cost safe solution. I read sugar is used in the mix. Honey could possibly replace that.
If honey is used in tissue culture, sugar could still be used in the mix. The article about Manuka Honey at althealthworks.com/9387/mysterious-honey-discovered-that-kills-all-bacteria-scientists-throw-at-it/ suggests it is very good and better than normal honey and "is capable of killing just about everything scientists throw at it, including some of the worst bacteria known to man". So only a small percentage of the tissue culture mix may need to include Manuka Honey for it to be effective and to replace the much more expensive PPM. It may take experimenting with a few different ratios to work out the best mix but it could be worth it as it could not only save a lot of money but could also give a better strike rate with less time involved for sterilizing. We could encourage a few different people to try it at different ratios.
I am planning on growing Venus Fly Traps for a start. I heard they can still be grown in tissue culture without hormones.
Do you know anyone that is interested in experimenting?
Regards Richard.
Do you know anyone that has tried honey in the tissue culture medium or are you able to try it? It kills bacteria and is good for plants and striking cuttings. I have used it to strike cuttings. Honey may increase the chances of the plants in culture living without infection and it may save a lot of time and expense of the extra sterilization. I thought it would be a low cost safe solution. I read sugar is used in the mix. Honey could possibly replace that.
If honey is used in tissue culture, sugar could still be used in the mix. The article about Manuka Honey at althealthworks.com/9387/mysterious-honey-discovered-that-kills-all-bacteria-scientists-throw-at-it/ suggests it is very good and better than normal honey and "is capable of killing just about everything scientists throw at it, including some of the worst bacteria known to man". So only a small percentage of the tissue culture mix may need to include Manuka Honey for it to be effective and to replace the much more expensive PPM. It may take experimenting with a few different ratios to work out the best mix but it could be worth it as it could not only save a lot of money but could also give a better strike rate with less time involved for sterilizing. We could encourage a few different people to try it at different ratios.
I am planning on growing Venus Fly Traps for a start. I heard they can still be grown in tissue culture without hormones.
Do you know anyone that is interested in experimenting?
Regards Richard.
