Post by DroseraBug on Jan 15, 2012 2:45:10 GMT
I've been sterilizing Sarracenia seed and germinating in-vitro for a while now. I'm looking for any tips on media prep for Sarracenia. Currenty I'm using 1/2 MS just because I prefer that with vfts, sundews, and pings.
Sarracenia seedling growing on 1/2 MS with no cytokinins or auxins
Another seedling growing on both cytokinins and auxins. I like the way these are growing a little better. They are so much slower than the vfts. But I'm definitely going to use auxins from now on. Auxin does seem to initiate some roots, shoots, and size. I'm looking for any tips on Sarracenia micropropagation. I'm using 1/2 MS for everything. I'm thinking I should reduce to 1/3 MS for Sarracenia only. Both of the above have been replated twice. I placed the last of the two on both hormones because I was interested in multiplication and root/shoot growth. With vfts cytokinins slow growth an really aren't even needed unless you want large quantities. I would assume cytokinins would also slow growth with Sarracenia however help to initiate callus or multiplication. I've done a good bit of research and found very little on this topic. Notice the very bulbous growth on the root apical meristems on the last photo compared to the first photo. The hybrid in the top photo was replated a week prior to the hybrid in the second photo. I'm thinking if I remove the cytokinins and use auxins only and 1/2 or 1/3 MS it may help with faster growth. Any advice?
Sarracenia seedling growing on 1/2 MS with no cytokinins or auxins
Another seedling growing on both cytokinins and auxins. I like the way these are growing a little better. They are so much slower than the vfts. But I'm definitely going to use auxins from now on. Auxin does seem to initiate some roots, shoots, and size. I'm looking for any tips on Sarracenia micropropagation. I'm using 1/2 MS for everything. I'm thinking I should reduce to 1/3 MS for Sarracenia only. Both of the above have been replated twice. I placed the last of the two on both hormones because I was interested in multiplication and root/shoot growth. With vfts cytokinins slow growth an really aren't even needed unless you want large quantities. I would assume cytokinins would also slow growth with Sarracenia however help to initiate callus or multiplication. I've done a good bit of research and found very little on this topic. Notice the very bulbous growth on the root apical meristems on the last photo compared to the first photo. The hybrid in the top photo was replated a week prior to the hybrid in the second photo. I'm thinking if I remove the cytokinins and use auxins only and 1/2 or 1/3 MS it may help with faster growth. Any advice?
