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Post by av8tor1 on Nov 21, 2011 20:24:10 GMT
Heliamphora folliculata pollen tubes (please forgive the poor technique, Im learning) thanks for looking, Av
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Post by Brian Barnes on Nov 22, 2011 14:18:36 GMT
Hey Butch, Really cool photos! I look forward to more... thanks for sharing with us. Happy Heli-growing, Brian
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Post by av8tor1 on Nov 22, 2011 15:59:33 GMT
Hey Butch, Really cool photos! I look forward to more... thanks for sharing with us. Happy Heli-growing, Brian Thanks Brian, fun project.... I hope to determine the "shelf life" of heli pollen and the effectiveness of common storage techniques
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Post by av8tor1 on Nov 30, 2011 19:10:40 GMT
Sample dried for two days in fridge using desiccant then froze for 7 days... thawed and checked for tube growth.
Growth observed
Obvious next question is just how long can it be stored using this method.
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Post by Brian Barnes on Dec 1, 2011 15:14:26 GMT
This is great information for any serious Heliamphora grower and hybridizer. Thanks Av for sharing your results! Please keep us updated... Happy Growing, Brian
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Post by Michael on Dec 2, 2011 2:00:22 GMT
I agree with Brian - Very Interesting Av!
Hopefully you'll do a write up and send it to the CPN!
Mike
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Post by av8tor1 on Dec 2, 2011 13:55:45 GMT
Brian, Mike
Thanks for the kind words.... Mike, I am honoured.... I will endeavor to put something together.
Butch
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Post by bluemax on Dec 4, 2011 8:32:03 GMT
Butch - interesting stuff! How are you inducing the pollen to grow tubules?
- Mark
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Post by av8tor1 on Dec 4, 2011 15:07:50 GMT
Mark,
Thanks.... I think these may be the first published images/video of Heliaphora pollen tube growth.
They basically take off themselves given a suitable growth media and enviroment. "Suitable growth media" is a variable I am working to improve on.
I am trying to come up with a Sucrose based formula. The images above were taken using the epidermis of a Spanish onion.
Butch
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Post by av8tor1 on Jul 7, 2013 1:36:37 GMT
update: February of 2012, I froze samples from 3 different species. Tested today (July, 2013) via pollen tube growth, all still viable after being frozen for 16m in a home refrigerator/freezer Sample #1 Av
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coline
Full Member
Life's essence: patience
Posts: 484
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Post by coline on Jul 7, 2013 12:33:34 GMT
What is the procedure you followed to freeze them?
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Post by av8tor1 on Jul 7, 2013 23:39:42 GMT
Coline,
Bear with me a little bit, I'm going to post a thread on the subject soon. Working on some new protocols.
Av
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Post by av8tor1 on Jul 9, 2013 16:37:59 GMT
still playing with old pollen :-) some cool looking 16m old pollen pics: thanks for looking
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Post by av8tor1 on Jul 9, 2013 18:29:16 GMT
Just took a peek at a killed (negative control) and non killed pollen sample Pollen had been frozen for 16m in standard residential refrigerator/freezer Verified viable by pollen tube growth Same pollen sample, same 0.1% MTT stain solution, and samples incubated together. First image is killed pollen, second one is non killed pollen It looks like staining with 0.1% MTT is a good indicator of pollen viability
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